ISO 16187 pdf download

ISO 16187 pdf download

ISO 16187 pdf download Footwear and footwear components — Test method to assess antibacterial activity
This International Standard specifies quantitative test methods to evaluate the antibacterial activity offootwear and components.
This International Standard is applicable to all types of footwear and components employing non-diffusing antibacterial treatments.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and areindispensable for its application, For dated references, only the edition cited applies. For undatedreferences, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use – Specification and test methods
ISO 19952,Footwear- Vocabulary
3 Terms and definitions
For the purposes ofthis document, the terms and definitions given in ISO 19952 and the following apply.
antibacterialactivityefficacy of a material or finish used to prevent or mitigate the growth of bacteria, to reduce the numberof bacteria or to kill bacteria
control samplematerial identical to the test material but without antibacterial treatment
Handling of microorganisms which are potentially hazardous requires a high degree of technicacompetence and can be subject to current national legislation and regulations. Only personnel trainedin microbiological techniques should carry out such tests. Codes of practice for disinfection, sterilizationand personal hygiene shall be strictly observed
NOTEltis recommended that workers consultIEC 60068-2-10,appendixA”Danger to personnel”andISO 7218.
5 Apparatus and materials
5.1? General
Standard laboratory equipment and the following.
5.2? Biological safety cabinet.
5.3? Incubator, capable of maintaining a temperature of (37 ± 2) °C.
5.4? Autoclave.
5.5? Humidity chamber, capable of maintaining a temperature of (37 ± 2) °C and a relative humidity not less than 90 %.
5.6? Ultraviolet lamp.
5.7? Wide mouth jars, with cap, 100 ml, capable of being used with an autoclave (5.4).
5.8? Cover? film that does not affect bacterial growth or absorb water, which can be made of either polyethylene, polypropylene or polyester [poly (ethylene terephthalate)]. Film that is 0,05 mm to 0,10 mm thick is recommended. For example, disposal bag suitable for use with an autoclave (5.4).
5.9? Vortex mixer.
5.10? Dimensional shaker, two dimensional or three dimensional, capable of adjusting to 50 rpm.
5.11? Shaking incubator, capable of maintaining a temperature of (37 ± 2) °C and a rotational
frequency of (120 ± 10) rpm.
6 Reagents and culture medium
6.1? Principle
The preparation and test shall be freshly prepared in order to ensure the culture quality.
NOTE This can be done according to ISO/TS 11133-1, ISO/TS 11133-2, or according to national standards or regulations.
Reagents used in tests shall be of analytical grade and/or suited for microbiological purposes.
Use only water Grade 3 according to ISO 3696.
6.2? Nutrient broth (NB)
6.2.1? Composition
Beef extract, 3,0 g.
Peptone, 5,0 g.
Sodium chloride (NaCl), 5,0 g.
Water, 1 000 ml.

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