ISO 13307 pdf download Microbiology of food and animal feed — Primary production stage — Sampling techniques
ThisInternationalStandard specifies sampling techniques within the primaryfood-animal production stagefor detection or enumeration ofviable microorganisms with particular reference to food-borne pathogens.
This International Standard is not intended for use in diagnosis of animal disease
2 Normative references
The following referenced documents are indispensable for the application of this document. For datedreferences, only the edition cited applies. For undated references, the latest edition of the referenceddocument (including any amendments) applies.
ISO 6887-1, Microbiology offood and animal feeding stuffs — Preparation oftest samples, initial suspensionand decimal dilutions for microbiological examination — Part 1: General rules for the preparation of theinitial suspension and decimal dilutions
ISO 7218,Microbiology of food and animal feeding stuffs – General requirements and guidance formicrobiologicalexaminations
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
primary production stageincludes all the stages of food production from farm until harvest or entry to the slaughterhouse3.2laboratory samplesample prepared for sending to the laboratory and intended for inspection or testing
The parties concerned or their representatives may be given the opportunity to be present whensamplingis performed.
Whenever special, e.g statutory, requirements are given for the sampling and/or arise from a specificanalysis to be performed, these requirements shall be followed.
4.2 Sampling personnel
Sampling for microbiological examination shall always be undertaken by a person trained andexperienced in the technique of sampling for microbiological purposes.
4.3 Packing and labelling ofsamples
Samples shall be packed in order to avoid cross-contamination and to prevent leakage orloss of moisture.
They shall be clearly identified.
The minimum details that shall accompany the samples are: the nature of the matrix, its identification,the name or initials of the person responsible for taking the samples, as well as the date, time (ifappropriate), and place of sampling.
This information should be recorded on a form. One form can be used for several samples provided eachhas unique identification and the samples are accompanied by the sampling form which lists the sampledetails with their unique identifying codes.
4.4 Preparation of a sampling form
Samples shall be accompanied by a report, ideally completed on a standard form provided by thelaboratory, signed orinitialled by the sampling personnel.The report shall give the following particulars:
— the place, date and time (if appropriate)of sampling;
— the names of the sampling personnel;
— the nature, number, and identity of samples constituting the consignment;
— the purpose of sampling and the microorganisms to be sought.
When appropriate, the report shall also include any relevant conditions or circumstances, and any special information relating to the product being sampled, e.g. difficulty in achieving representative samples.
If any additives such as diluents, transport media or neutralizing agents are used, these shall be recorded.
5 Diluents and disinfectants
5.1.1 General. Diluent used for moistening all kind of swabs (bootswabs, stick swabs etc.):
— peptone salt solution prepared according to ISO 6887-1;
— buffered peptone water prepared according to ISO 6887-1;
— sterile water;
— potable water for samples where this would not interfere with the analysis, e.g. bootswabs.